1. What is DNA Copy Number Calculation?

The DNA copy number calculation determines the number of molecules in a given mass of DNA based on its molecular weight. This is essential for molecular biology applications like PCR, sequencing, and cloning.

2. How Does the Calculator Work?

The calculator uses the copy number equation:

Where:

• \( CN \) — Copy number (dimensionless)
• \( C \) — Concentration in grams (g)
• \( MW \) — Molecular weight in grams per mole (g/mol)
• \( 6.022 \times 10^{23} \) — Avogadro's number (molecules/mol)

Explanation: The equation converts mass of DNA to number of molecules using Avogadro's number and the molecular weight of the DNA.

3. Importance of Copy Number Calculation

Details: Accurate copy number calculation is crucial for preparing samples with specific molecule counts, ensuring consistency in molecular biology experiments.

4. Using the Calculator

Tips: Enter concentration in grams and molecular weight in g/mol. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why is Avogadro's number used in this calculation?
A: Avogadro's number converts between moles (a chemical quantity) and individual molecules.

Q2: How do I determine molecular weight of my DNA?
A: For double-stranded DNA, MW ≈ (number of base pairs) × 650 g/mol/bp. For single-stranded DNA, use sequence-based calculators.

Q3: What concentration units should I use?
A: The calculator requires grams. If you have ng/μL, convert to grams by multiplying by 10^-9 and your sample volume.

Q4: Can this be used for RNA or proteins?
A: Yes, the same equation applies to any molecule when you know its molecular weight.

Q5: What's a typical copy number range for PCR?
A: Common template amounts range from 10^3 to 10^6 copies per reaction, depending on application.