1. What is DNA Molarity?

DNA molarity refers to the concentration of DNA in solution expressed in moles per liter (mol/L). It's calculated by dividing the mass concentration (g/L) by the molecular weight (g/mol) of the DNA.

2. How Does the Calculator Work?

The calculator uses the molarity equation:

Where:

• \( M \) — Molarity (mol/L)
• \( conc \) — DNA concentration (g/L)
• \( MW \) — Molecular weight (g/mol)

Explanation: The equation converts mass concentration to molar concentration by accounting for the molecular weight of the DNA.

3. Importance of DNA Molarity Calculation

Details: Knowing the molar concentration of DNA is essential for molecular biology experiments like PCR, sequencing, and cloning where precise amounts of DNA are required.

4. Using the Calculator

Tips: Enter DNA concentration in g/L and molecular weight in g/mol. Both values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: How do I determine the molecular weight of my DNA?
A: For double-stranded DNA, MW ≈ 660 g/mol per base pair. For single-stranded DNA, MW ≈ 330 g/mol per nucleotide.

Q2: What's the difference between ng/μL and g/L?
A: 1 ng/μL = 1 mg/L = 0.001 g/L. Convert your units before using the calculator.

Q3: Why is molarity important in PCR?
A: PCR requires optimal primer and template concentrations, typically expressed in molar terms (nM or μM).

Q4: Does this work for RNA as well?
A: Yes, the same equation applies to RNA molarity calculations.

Q5: How accurate is this calculation?
A: The calculation is mathematically precise, but accuracy depends on your input measurements of concentration and molecular weight.